Rapid and efficient organism identification and antimicrobial susceptibility reports are paramount for patient survival during sepsis. The Bruker MBT Sepsityper® Kit US IVD allows harvesting of microorganisms directly from positive blood cultures and pairs it with the Bruker MALDI Biotyper® CA System for rapid identification of bacterial isolates using MALDI-TOF technology. Here we compare the accuracy of the MBT Sepsityper® Kit US IVD for bacterial identification to culture-based methods. Further, as an off-label use of the kit, we compare the antimicrobial susceptibility results for Gram-negative bacteria setup directly using the harvested pellet to the established method in the laboratory.