Expert Balance
Expertly balances clinical utility and ease of use
Screen, identify & semi-quantify Known & Unknown targets with much more confidence
Identified Polar, a nonpolar compound in a single run
>12 min Run & Analysis time (Sample to Report)
Curated Library & Methods for over 6000+ Toxicology relevant compounds & their metabolites
New compound can be added in few clicks
UHPLC combined with true MSn and comprehensive Drug Libraries
Features of QF PCR
Single Report
Single reports using GeneMarker™ and GeneMapper® software applications
Simple Analysis
Simple analysis and all validated kits.
Set
Simple, Single tube reaction set.
Benefits of QF PCR
Easy determination of chromosome specific STR
The observation of an extra STR allele as either a three peak pattern in a 1:1:1 ratio or two peak pattern in a 2:1 or 1:2 peak ratio is diagnostic of the presence of an additional sequence, which in turn may represent an additional chromosome as in the case of a trisomy.
Least Turnaround Time
Turnaround reporting times of less than 24 hours from sample receipt are easily achievable
Best Compatibility with most systems
It is compatible with most commonly employed DNA extraction protocols, thermal cyclers and capillary electrophoresis instruments.
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An instrument for every need:
Integrated benchtop system for Research Labs
For Core Labs and High Sample Throughput
nCounter Life Science Assays:
nCounter Elements™:
Expandable with Additional Prep Station:
Enterprise Package:
PROSIGNA optional add-on:
Runs Per Day:
Throughput (Lanes Per Day):
Hands on Time:
Reaction Volume Required:
Linear Dynamic Range:
Throughput:
Yes
Yes
No
No
No
2
24
10 mins
Up to 35 µl
6 x10* total count
12 lanes/6 hours
Yes
Yes
Yes
Yes
No
4*
48-96*
15 mins
Up to 30 µl
7 x10* total count
12 lanes/2.5 hours
nCounter Sprint
nCounter Flex
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UHPLC combined with true MSn and comprehensive drug libraries
Related Resources
Powerful integration:
The IR Biotyper and Bruker’s MALDI Biotyper can now be combined into a single seamless workflow. Data from the MALDI Biotyper® – which uses MALDI-TOF MS to identify microorganisms to species or genus level within a few minutes – can be imported into the IR Biotyper software, and once analyzed, the entire set of results can be exported to the laboratory’s LIMS in CSV format.
Simple Workflows for Rapid Processing
Analysis by DART-MS relies on a gas-phase ionization mechanism. Initial generation of the ionizing species is by a corona discharge with helium or nitrogen which delivers excited gas atoms that, upon their release into the atmosphere, initiates a cascade of gas-phase reactions. This results in reagent ions created from atmospheric water or (solvent) vapor in the vicinity of the surface subject to analysis where they affect a chemical ionization process. DART ionization processes can generate positive or negative ions, predominantly even-electron specie
Comprehensive Solutions for Every Workflow
Quantitative fluorescent polymerase chain reaction (QF-PCR) can be applied to the detection of chromosome copy number by amplification of repeat sequences at polymorphic loci. These repeat sequences are amplified by PCR, and the labelled products are separated by gel electrophoresis.
An allele pattern of two equal peaks within the same chromosomal region is diagnostic of two copies of the target region, whereas three peaks within the same chromosomal region or two peaks with a ratio of 2 : 1 are indicative of trisomy for the target region. This diagnostic approach was first suggested in 1993, and prospective studies were carried out in the mid 1990s, followed by the development of a QF-PCR-based test for sex chromosome imbalance.
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