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Rapid Protein Sample Preparation Kits

Rapid Protein Sample Preparation Kits

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Screen, identify & semi-quantify Known & Unknown targets with much more confidence

Identified Polar, a nonpolar compound in a single run

>12 min Run & Analysis time (Sample to Report)

Curated Library & Methods for over 6000+ Toxicology relevant compounds & their metabolites

 

New compound can be added in few clicks

UHPLC combined with true MSn and comprehensive Drug Libraries

Streamline your protein sample preparation by using iST Kits

Rapid Methods

Rapid Methods

Rapid methods employ optimized digestion conditions, including the use of specialized enzymes, to achieve rapid and complete digestion, reducing digestion times from hours to minutes.

iST Method

iST Method

The iST method provides significant time savings as complex workflows previously requiring many different reagents taking 48 hours are replaced by a single kit containing all standardized reagents.

iST Kit

iST Kit

The iST kit allows proteomics sample preparation for mass spectrometry analysis, whether manually, semi or fully automated in a 2 hour time window.

Step by step iST workflow for mammalian tissues
Step by step iST workflow for plasma and serum
Step by step iST workflow to process plant samples
Plant samples: optimized processing with the iST workflow

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Featured Applications

An instrument for every need:

Integrated benchtop system for Research Labs

For Core Labs and High Sample Throughput

nCounter Life Science Assays:

nCounter Elements™:

Expandable with Additional Prep Station:

Enterprise Package:

PROSIGNA optional add-on:

Runs Per Day:

Throughput (Lanes Per Day):

Hands on Time:

Reaction Volume Required:

Linear Dynamic Range:

Throughput:

Yes

Yes

No

No

No

2

24

10 mins

Up to 35 µl

6 x10* total count

12 lanes/6 hours

Yes

Yes

Yes

Yes

No

4*

48-96*

15 mins

Up to 30 µl

7 x10* total count

12 lanes/2.5 hours

nCounter Sprint
nCounter Flex

Product Comparison

Related Products

Assays/Consumables/Software

  • UHR QTOF mass spectrometer with Full Scan MS and broadband CID (bbCID) MS/MS methods

  • An Elute UHPLC system, with Bruker columns, mobile phases, and QC standard

  • TASQ screening and quantitation software for rapid data processing, including ready methods for multi-target screening

  • The high-quality and robust TargetScreener database including more than 3000 entries relevant for food safety, environmental protection, and toxicology research screening

UHPLC combined with true MSn and comprehensive drug libraries

Powerful integration:

The IR Biotyper and Bruker’s MALDI Biotyper can now be combined into a single seamless workflow. Data from the MALDI Biotyper® – which uses MALDI-TOF MS to identify microorganisms to species or genus level within a few minutes – can be imported into the IR Biotyper software, and once analyzed, the entire set of results can be exported to the laboratory’s LIMS in CSV format.

Simple Workflows for Rapid Processing

iST Kit for Rapid Protein Sample Preparation

Analysis by DART-MS relies on a gas-phase ionization mechanism. Initial generation of the ionizing species is by a corona discharge with helium or nitrogen which delivers excited gas atoms that, upon their release into the atmosphere, initiates a cascade of gas-phase reactions. This results in reagent ions created from atmospheric water or (solvent) vapor in the vicinity of the surface subject to analysis where they affect a chemical ionization process. DART ionization processes can generate positive or negative ions, predominantly even-electron specie

Comprehensive Solutions for Every Workflow

Liquid chromatography-mass spectrometry (LC-MS) has revolutionized proteomics research by enabling the identification and quantification of proteins in complex biological samples. However, traditional protein sample preparation methods can be time-consuming and labor-intensive, posing significant challenges when dealing with large sample cohorts or time-sensitive experiments. In response to these demands, rapid protein sample preparation techniques have emerged, offering researchers efficient and streamlined workflows without compromising data quality.


The challenges of traditional protein sample preparation: 

  • Traditional protein sample preparation typically involves several steps, including cell lysis, protein extraction, protein purification, and enzymatic digestion. 

  • While these methods have been widely used and validated, they often require extensive manual labor, are prone to sample loss or contamination, and can be time-consuming, limiting the throughput and efficiency of protein analysis workflows. 

  • Additionally, the use of harsh chemicals and multiple sample handling steps can introduce variability and affect protein stability, potentially impacting downstream LC-MS analysis results.

  • Preparing labelled samples over an extended time period with a complex method leads to variation in results. 

  • This challenge is exacerbated with large numbers of samples and small starting amount of input material.

Rapid Protein Sample Preparation Kits

Rapid Protein Sample Preparation Kits

Enquire Now
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