Benefits of timsTOF
PASEF enables MS/MS acquisition at >100 Hz, making high-throughput measurements using short gradients possible while maintaining deep proteome coverage or high protein depth measurements in less time.
It identifies more near-isobaric peptides and post-translationally modified (PTMs) peptides per unit time. PTM-positional isomers can also be quantified.
CCS-Aware analysis software increases the confidence of identifications and quantitation, while increasing data completeness.
The time-focusing benefit of dual-TIMS funnel technology increases sensitivity thereby allowing for loading of lower sample amounts and simpler sample preparation techniques.
The orthogonal geometry of the inlet glass capillary to the axis of the mass analyzer combined with dual-TIMS funnel technology, greatly increases robustness, allowing for sustained MS performance over large sample cohorts, maximizing instrument uptime.
Open data format easily allows timsTOF data to be used in custom developed bioinformatics solutions.
Analyze isomeric compounds
High-confidence forensic screening
Characterize metabolites and secondary metabolites
True Isotopic Pattern or TIPTM
Food and environmental research
Polymer analysis
Applications
Features
Resolution
Consistently high ion mobility resolution unravels the complexity of samples and unlocks insights that remain hidden in standard mass spectrometry
Flexibility
Adjustable ion mobility separation based on TIMS allows the exploration of samples in diverse and meaningful ways
Confidence
Boosts confidence and specificity of compound identification with an expanded set of orthogonal qualifiers that are measured with great accuracy
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Workflow of the System
Introducing Bruker's timsTOF - Next generation ion mobility separation
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The highly unique TIMS design enables unmatched duty cycle (up to 100%), significant improvement in IMS resolution (R>200), unprecedented collisional cross section (CCS) reproducibility and high sensitivity.
timsTOF combines high ion mobility resolution with ultra-high resolution QTOF technology for optimal performance. imeXTM technology further boosts the functionality by making ion mobility resolution an adjustable parameter.
Investigating samples in imeX survey mode can lead to mobility-resolved full scans, and then extending workflow with a detailed examination of selected compounds and precise collisional cross sections (CCS). Increased ion mobility resolution up to 200 for unique insights into samples can be acheived in imeX ultra mode.
Data-independent acquisition dia-PASEF is both more sensitive and selective than traditional DIA approaches as it applies the PASEF principle to combine the advantages of DIA with the inherent ion efficiency of PASEF. Over the entire liquid chromatography-mass spectrometry (LC-TIMS-MS)/MS dia-PASEF run, a perfect data cuboid is created containing m/z, ion mobility (CCS), retention time and intensity. TIMS separation increases selectivity, excludes singly charged precursors from fragmentation and cleans up the sample by concentrating signals from noise. Making use of the correlation of molecular weight and CCS coded information from the dual-TIMS funnel, dia-PASEF enables highly confident identification.
The timsTOF fleX combines 4D-Omics with MALDI Imaging technology, including smartbeam 3D laser optics to be a fast measurement all in one platform.
It is a dual source instrument ideal for SpatialOMx®. It conducts robust ESI measurements and spatially resolves a wide range of molecules directly from tissue using one platform.
During operation, software activation of the smartbeam 3D laser is the only change in the source region to change from ESI to MALDI in seconds.
timsTOF Flex MALDI 2 provides post ionization technique which reduces ion suppression effects and improves sensitivity by orders of magnitude. After the initial MALDI process, a second laser, sitting parallel to the sample surface, fires into the evolving plume and post-ionizes neutral (mainly matrix) molecules. A charge transfer from post-ionized matrix molecules to neutral analyte molecules leads to an amazing sensitivity gain for many analytes.
MALDI-2 post-ionization compensates the lower sample amount per pixel, making even the finest molecular distributions visible. Combining MALDI-2 post-ionization with microGRID enabled high spatial resolution opens the field for the analysis of the smallest unit of eukaryotic life - single cells.
TimsTOF MALDI Pharmapulse (MPP) uses a dual MALDI / ESI ion source with industry-leading 10 kHz smartbeam™ 3D laser which enable uHTS compatible speed and throughput. It supports rapid gas-phase separation of isobars, and even isomers, by exploiting the molecular collisional cross-section. This, in combination with routine 50,000 mass resolution in QTOF-MS detection facilitates revolutionary levels of assay specificity. It features the extreme speed and proven robustness of MALDI in the essence of tims technology.
It has an automation interface which supports HTS application of drug discovery. It has MPP 2023 which enables seamless transfer of data and results to downstream analysis software.
Faster separation of isobars and isomers
It is capable of separating isobars and even isomers on a timescale of ≤ 1 sec per separation cycle.
Data quality delivered at uncompromised HTS speed
Readout of a 1536 formatted sample plate in high-resolution MS or MS/MS mode takes less than 10 minutes
Related Resources
DART-OS enables a very simple workflow, for a wide range of sample types
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Start the system - switch on the source and start the VAPUR pump
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Spot or dose the sample onto an OpenSpot® sample card in the clearly marked area
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Slot the card into the system to guide your sample into exactly the right position for successful analysis
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Scan – the system will apply transmission mode DART passing a heated carrier gas through the sample
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Remove the card and discard